Laboratory
Safety
General
Rules and Regulations
To reduce the microbial population in
the laboratory equipments at all times by the following steps.
1.While
entering the laboratory, place the coats, note books and other belongings in
allotted space like shelves or cupboard, should not place on the working
tables.
2.During
the laboratory working time, close the windows and doors to prevent the
contamination.
3.At
the beginning and end of the laboratory session, clean the working tables and
the equipments by using the disinfectants
4.Don’t
keep the contaminated equipments like, inoculating loops, needles, and
pipettes, on bench tables. Contaminated instruments should be disposing or
sterilized properly.
5.At
the end of the lab session, all the microbial cultures and other materials
should be placed in specified area in laboratory. Fungal cultures were disposed
rapidly to prevent the dissemination in the lab environment.
The following regulations should be
observed to avoid the accidental injury and any infection of yourself and
others.
1.Upon
entering and before you leaving the laboratory, clean your hands with
disinfectants (95% ethyl alcohol).
2.Always
wear the lab coat while working in the laboratory to prevent the contamination
and discoloration by staining or other chemical solutions.
3.While
you performing the lab experiments, wear the gloves to avoid the microbial
contamination and accidental spilling of stains and other chemical solutions.
4.Use
the mask, paper cap and safety goggles to prevent your face, hair and eyes from
the microbes and other laboratory chemicals.
5.Wear
laboratory shoes at all times in the laboratory environment.
6.Avoid
applying cosmetics or inserting contact lenses inside the laboratory.
7.To
prevent or reduce the contamination, do not eat or drink inside the laboratory
and no mouth pipetting.
8.
Always use test tube stand/rack for carrying the microbial cultures, when
moving around the laboratory. And keep the cultures in test tube stand/rack on
working table when not in use.
9.
Avoid the unnecessary conversation and movements inside the laboratory that may
prevent disturbance.
10. Upon
leaving the laboratory, check the equipments are in the proper location and gas,
water and Bunsen burner were turned off.
11. Use
the waterproof marker and small roll of masking tape to clearly label your
cultures.
12. If
you spill or drop the culture remove properly like, cover paper towel over and saturate
the disinfectant over the towel. Kept for 15 minutes and then clean with fresh
paper towels. Should discard the contaminated paper towels in the proper
receptacle and clean your hands carefully.
13. Unnecessarily,
do not open Petri dishes in the lab to prevent/reduce the contamination.
14. Always
keep the culture tubes (broth and slants) in upright position in rack and the basket
for incubation or disposal.
15. Use
the solid waste materials in biohazard bag and should autoclave it prior to
discarding.
16. Treat
all cultures as potentially pathogenic, i.e., flood areas with disinfectant if
cultures are spilled, wash hands after contact and notify your instructor at
once.
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